C.V

 

   Name : Ashraf Mohamed Mohamed Mahmoud Sayed

 

Nationality

 Egyptian

Gender

Male

   

Date of Birth

May  01, 1969

Place of Birth

Assiut - Egypt

Social status

Married + 3 sons

College

Pharmacy

Department

Pharmaceutical Chemistry

   

Position

In Egypt : Professor in the Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University

In KSA   : Professor in the Department of Pharmaceutical  Chemistry, Faculty of Pharmacy, Najran University

Date of Latest degree

Ph.D. from Freie University, Berlin (Germany), May 2005.

Address    

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy,  Assiut University,  Assiut 71526, Egypt.

Degrees and Qualifications

1. Ph.D. in Pharmaceutical Analytical Chemistry. "Radioimmunoassay and radiochemical procedure for determination of cutaneous uptake and metabolism of sex steroid hormones" from Freie University, Berlin (Germany), May 2005.

2.  M.Sc. in Pharmaceutical Analytical Chemistry.  "Analysis of some antifungal drugs in pure forms and in pharmaceutical preparations".   Assiut University, Egypt.   June   1998.

3. B.Sc. in Pharmaceutical Science. Very Good with Degree of Honour.  Assiut University, Egypt.  May, 1992.

History of academic and employment career

1.   Professor in the Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Najran University, KSA.  Sept., 2016  to  date (College coordinator since Juli 2012, Head of academic office in medicinal colleges since Oct. 2012, Academic Advisor of Pharmacy since 2012)         

2.   Associate Professor in the Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Najran University, KSA.  Sept., 2011  to  Sept., 2016 

3.  Associate Professor in the Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Egypt.  Sept., 2010  to  Sept., 2016 

4.  Lecturer in the Department of Pharmaceutical Analytical Chemistry,                                    Faculty of Pharmacy, Assiut University, Egypt.   Jul. 31st  , 2005  - Jul. 31st  2010

5.  Assistant professor in the Department of Pharmaceutical Chemistry,                                    College of Pharmacy, King Saud University, Saudi Arabia. Sept. 6th 2006 – Jul.15th  2009.

6.  Assistant Lecturer, in the Department of Pharmaceutical Analytical Chemistry,                                    Faculty of Pharmacy, Assiut University, Egypt.,   Aug., 15th 1998 –Jul., 30th 2005.

7.  Demonstrator, in the same department.  Jan., 15th 1993 – Aug., 15th 1998.

 Grants, and Prizes

1.   Scholarship for Studying of Ph.D. in Berlin, Germany from the Ministry of High Education, Egypt,  April 28th , 2000 – May, 31st  2005.

2.  Assiut University Encouragement Award for Research in Medicinal and Pharmaceutical Fields  2011-2012

Teaching experience

1.        External Evaluator and Examiner in Faculties of Pharmacy of Cairo, El-minia, El-Mansoura, Al-Azhar, Russian and El-Nahda-Beny Swief universities.

2.        Teaching of quantitative analytical chemistry courses for undergraduate pharmacy students in Assiut, Al-Azhar and King Saud Universities

3.        Teaching of qualitative analytical chemistry courses for undergraduate pharmacy students in Al-Azhar University, Assiut branch.

4.        Teaching of instrumental analysis (Chromatography [HPLC, GC, HPTLC], electrophoresis, UV-VIS spectrophotometry,  fluorimetry, and chemoluminescence) courses for undergraduate pharmacy students in Assiut University.

5.        Teaching of quality control & pharmaceutical analysis course for undergraduate clinical pharmacy students in Assiut University.

6.        Teaching of advanced spectroscopic methods (UV-VIS, IR, Mass, 1H-NMR, and atomic spectroscopy) course for undergraduate clinical pharmacy and postgraduate students in Assiut University.

7.        Teaching of Advanced spectroscopic analysis and extraction & clean up methodology courses for Ph.D. pharmacy students in Assiut University.

8.        Teaching of Advanced instrumental analysis courses for M.Sc. pharmacy students (PHC 516, 521, 523), King Saud University.

9.        Teaching of kinetics methods in pharmaceutical analysis for M.Sc. pharmacy students, King Saud University.

10.    Teaching of Pharmacoepial methods of pharmaceutical analysis for undergraduate pharmacy students, King Saud University.

11.    Teaching of pharmaceutical analysis of drugs for undergraduate pharmacy students in King Saud University.

12.    Teaching of quantitative analytical chemistry courses for undergraduate pharmacy students in Najran Universitiy

13.    Teaching of qualitative analytical chemistry courses for undergraduate pharmacy students in Najran Universitiy.

14.    Teaching of instrumental analysis (Chromatography [HPLC, GC, TLC], UV-VIS spectrophotometry,  fluorimetry, electrochemistry and chemoluminescence) courses for undergraduate pharmacy students in in Najran University.

15.    Teaching of quality control of pharmaceutical preparations course for undergraduate pharmacy students in in Najran University.

Committees 

1.   Egyptian Syndicate of Pharmacists, July 1992 to date.

2.   Egyptian Society of Pharmacists, 1994 to date.

3.   Deutsche Society of Pharmacists, since 2003.

Conferences  and symposia

1.     Assiut University First Pharmaceutical Sciences Conference,
Assiut, Egypt, 6-7 Marsh, 1998.

2.      Deutsche Pharmazeutische Gesselschaft conference. Berlin, Germany,  9 (2002).
"Cutaneous estradiol permeation, penetration and metabolism in pig and man ''

         A Mahmoud, A Haberland, M Dürrfeld, B Christ, D Heydeck, M Sch?fer-Korting.

3.      Deutsche Pharmazeutische Gesselschaft conference, Wurzburg, Germany, 2003.

"Validation study for investigation of the penetration through skin models: First results S Schreiber, A Mahmoud, C Santos Maia, M K Rübbelke, M Schaller, H C Korting, M Sch?fer-Korting.

4.      Deutsche Forschung Gruppe Symposium, Berlin, Germany, 2004

"Validation study on percutaneous absorption via human skin models: Protocol transfer.  S Schreiber, A Mahmoud, M K Rübbelke, M Schaller, H C Korting, E Schmidt, M Liebsch, M Sch?fer-Korting

5.      Assiut University 5th Pharmaceutical Sciences Conference in Assiut, Egypt, Marsh 7-8, 2006 "Simple colorimetric analysis for H2-receptor antagonists by the reaction with n-bromosuccinimide and fluorescein''. S. Abdel-Rahman, I. Darwish, A. Mahmoud, A. Hassan

6.      The 7th International Saudi Pharmaceutical Conference Riyadh, Arabia Saudia, Marsh 19-21, 2007
"Development and validation of spectrofluorometric and spectrophotometric methods for determination of amlodipine in pharmaceutical dosage forms''
H M. Abdel-Wadood, N A. Mohamed, and A M. Mahmoud

7.      The Annual meeting of the Controlled Release Society (CRS)  Long Beach, USA, 7th-11th July, 2007 "Human Reconstructed Skin Models for Absorption Testing: Comparison of hydrocortisone and testosterone formulations"

S Schreiber, S Borgia, A Mahmoud, W Mehnert, B Kleuser, M Sch?fer-Korting

8.      The Fourth International Conference for development and the environment (F.I.C.D.E.) "The Balance between Development and Environment Conservation"

Riyadh, Arabia Saudia, Marsh 18-20, 2008

9.      1ST Workshop (Nano Research at Universities: The road toward fulfilling the vision of Custodian of the two Holy Mosques), Riyadh, Arabia Saudia, Oct 28-29, 2007

10.   Assiut University 7th Pharmaceutical Sciences Conference, Assiut, Egypt, Marsh 17-18th , 2010 "New sensitive HPLC method with automated on-line column switching and fluorescence detection for determination of sertraline in human plasma"  A. M. Mahmoud, N. Y. Khalil, I. A. Darwish, A. A. Al-Majed

11.   Assiut University 8th Pharmaceutical Sciences Conference

Assiut, Egypt, Marsh 14-15th, 2012  "Novel simple HPTLC approach to avert pretreatment of plasma samples: Application to the determination of gemifloxacin mesylate using fluorescence detection" A. M. Mahmoud, S.R. El-Shabouri, N.N. Atia, W.M. El-koussi

"New spectrofluorometric determination of gemifloxacin mesylate in spiked human plasma and pharmaceutical dosage forms using Hantzsch reaction"
N.N. Atia, A. M. Mahmoud, S.R. El-Shabouri, W.M. El-koussi

   

Publication and fields of  Researches of Interest

MAJOR RESEARCH/TECHNOLOGY TOPICS OF INTEREST

1.      Development and validation of HPLC methods for the analysis of the pharmaceutically active compounds and their metabolites in biological fluids.

2.      Development and validation of immunoassay methods for the analysis of pharmaceutical compounds and cancer markers in biological fluids.

3.      Development and validation of fluorimetric and spectrophotometric methods for the analysis of pharmaceutical compounds in pure forms and pharmaceutical dosage forms.

4.      In vitro validated alternatives to animal experiments and human volunteers for the development of topical dermatics and for studying the risk analysis.

5.      Percutaneous absorption from different skin models; Excised Human and Pig Skin, Reconstructed Epidermis, Full-Thickness Skin Models and Epiairway Models.

6.      Development or adopting, and validation of radioimmunoassay methods for the analysis of pharmaceutical compounds, steroidal hormones in biological fluids and skin tissues.

7.      Quality control for the analysis of drugs in dosage forms or/and in biological fluids.

 

RESEARCH PROJECTS

1.     "In vitro validated alternatives to animal experiments" research project which is financially supported by the German Validation Center for Alternative Methods (ZEBET; WK 1-1328-170), Berlin, Germany, 2005. The project evaluate the formulation effects on percutaneous absorption of different skin models; reconstructed epidermis and full-thickness skin models for reducing the need for animal experiments and the risks for patients receiving a new drug or new formulation.

2.      "Formal large scale validation study comparing the uptake of chemicals by reconstructed epidermis to uptake in human and animal skin to set up a standard protocol for the use of reconstructed epidermis in routine skin absorption tests" research project which is financially supported by the German Ministry of Education and Research (BEO 031288-1/4/6), Berlin, Germany, 2003.

3.      "Development of Sensitive Immunoanalytical Technique for 2'-Deoxycytidine as a Potential Prognostic Marker for Breast Cancer Chemotherapy." research project which is financially supported by the Research Center, College of Pharmacy, King Saud University, Saudi Arabia (grant No. CPRC. 183).

4.      "Synthesis and Investigation of Some Amantadine Derivatives as Hepatic Delivery System to Enhance its Activity against HCV." research project which is financially supported by the Research Center, College of Pharmacy, King Saud University, Saudi Arabia (grant No. CPRC. 181).

5.      "Novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis." research project which is financially supported by the Research Center, College of Pharmacy, King Saud University, Saudi Arabia (grant No. CPRC. 225).

SUPERVISION OF THESES

1.       Supervision of the M. Sc. Thesis "Analytical Methods for H2-Receptor Antagonists" Faculty of Pharmacy, Assiut University, Egypt. (finished, 2007)

2.      Supervision of M. Sc. thesis "New Selctive Analytical Methods for Determination of Gemifloxacin in pharmaceutical preparation and biological fluids" Faculty of Pharmacy,  Assiut University, Egypt. (finished, 2014)

3.      Supervision of Ph.D. thesis.  "Analysis of some pharmaceutical drugs and biologically active compounds using capillary electrochromatography" Faculty of Pharmacy, Assiut University, Egypt. (finished, 2014)

4.      Supervision of Ph.D. thesis "New HPLC and votammetric methods for determination of certain hypolipidemic drugs" Faculty of Pharmacy, Assiut University, Egypt. (under Supervision)

 

LIST OF PUBLICATIONS

 

1.     Salwa R El-Shabouri, Kamla M Emara, Pakinaz Y Khashaba, Ashraf M Mohamed: Charge-transfer complexation for spectrophotometric assay of certain imidazole antifungal drugs. Anal. Lett., 31 (1998)1367-1385.

2.     PY Khashaba, SR El-Shabouri, KM Emara, AM Mohamed: Analysis of some antifungal drugs by spectrophotometric and spectrofluorimetric methods in different pharmaceutical dosage forms. J. Pharm. Biomed. Anal., 22 (2000)363-76.

3.     A Mahmoud, A Haberland, M Dürrfeld, D Heydeck, S Wagner, M Sch?fer-Korting: Cutaneous estradiol permeation, penetration and metabolism in pig and man. Skin Pharmacology and Physiology, 18 (2005)27-35.

4.     S Schreiber, A Mahmoud, A Vuia, MK Rübbelke, E Schmidt, M Schaller, H Kand?rov?, A Haberland, UF Sch?fer, U Bock, HC Korting, M Liebsch, M Sch?fer-Korting: Reconstructed epidermis versus human and animal skin in skin absorption studies. Toxicology in Vitro, 19 (2005)813-822.

5.     Ibrahim A. Darwish, Ashraf M. Mahmoud, Abdel-Rahman A. Al-Majed: generic simple enzyme immunoassay approach to avert small molecule immobilization problems on solid phases. Application to the determination of tobramycin in serum. Talanta, 72 (2007)1322-1328.

6.     Ibrahim A. Darwish, Samiha A Hussein, Ashraf M Mahmoud, Ahmed I Hassan: Sensitive indirect spectrophotometric method for determination of H2-receptor antagonists in pharmaceutical formulations. Int. J. Biomed. Sci., 2007, 3: 123-130.

7.     Hanaa M. Abdel-Wadood, Niveen A. Mohamed, Ashraf M. Mahmoud: Validated spectrofluorometric methods for determination of amlodipine besylate in tablets. Spectrochimica Acta Part A, 70 (2008) 564-570.

8.     Ibrahim A. Darwish, Ashraf M. Mahmoud, Nasr Y. Khalil: Non-extractive procedure and pre-column derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole for trace determination of trimetazidine in plasma by HPLC with fluorescence detection J. AOAC Int., 91 (2008)1037-1044.

9.     Ibrahim A. Darwish, Samiha A Hussein, Ashraf M Mahmoud, Ahmed I Hassan: Spectrophotometric determination of H2-receptor antagonists via their oxidation with cerium (IV). Spectrochimica Acta Part A, 69 (2008)33-40.

10.  Monika Sch?fer-Korting, Ashraf Mahmoud, Simone Lombardi Borgia, Barbara Brüggener, Burkhard Kleuser, Sylvia Schreiber, Wolfgang Mehnert: Reconstructed Epidermis and Full-Thickness Skin for Absorption Testing: Influences of the Vehicles Used on Steroid Permeation. ATLA, 36 (2008) 441-452.

11.  Ibrahim A. Darwish, Samiha A Hussein, Ashraf M Mahmoud, Ahmed I Hassan: A sensitive spectrophotometric method for the determination of H2-receptor antagonists by means of N-bromosuccinimide and p-aminophenol. Acta Pharmaceutica, 58 (2008) 87-97.

12.  Ibrahim A. Darwish, Ashraf M. Mahmoud, Abdel-Rahman A. Al-Majed, Tarek Abo-ElFadl, Nasr Y. Khalil: A Highly Sensitive Enzyme Immunoassay For Evaluation Of 2'-Deoxycytidine Plasma Level As A Prognostic Marker For Breast Cancer Chemotherapy, Anal. Chim. Acta, 632 (2009) 266-271.

13.  Ibrahim A. Darwish, Abdel-Rahman A. Al-Majed, Ashraf M. Mahmoud, Nasr Y. Khalil: New non-extractive and highly sensitive HPLC method for determination of paroxetine in plasma after offline precolumn derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole. J. AOAC Int., 92 (2009)1-8.

14.  Ashraf M. Mahmoud, New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms. Int.J.Anal.Chem,2009 (2009)1-11 (Published online, doi:10.1155/2009/307045).

15.  Ashraf M. Mahmoud, Nasr Y. Khalil, Ibrahim A. Darwish, Tarek Aboul-Fadl: Selective spectrophotometric and spectrofluorometric methods for the determination of amantadine hydrochloride in capsules and plasma via derivatization with 1,2-naphthoquinone-4-sulphonate. Int. J. Ana.l Chem., 2009 (2009) (Published online, 8 pages, doi: 10.1155/2009/810104).

16.  Ashraf M. Mahmoud, Ibrahim A. Darwish, Nasr Y. Khalil: Fluorometric study for the reaction between sertraline and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: kinetics, mechanism and application for the determination of sertraline in tablets. J Fluorescence 20 (2010) 607–613.

17.  Nasr Y. Khalil, Ashraf M. Mahmoud, Ibrahim A. Darwish, Abdul-Rahman A. Al-Majed: "Highly Sensitive HPLC Method with Automated Co-Sense System and Fluorescence Detection for Determination of Sertraline in Human Plasma" Chromatographia 72 (2010).

18.  Ibrahim Darwish, Samiha Abdel-Rahman, Ashraf Mahmoud, Ahmed Hassan: "Spectral investigation and analytical application  of the vinylamino-substituted haloquinone derivatives of nizatidine and ranitidine" Int. J. Res. Pharm. Sci., 1 (2010) 1-7.

19.  Ibrahim Darwish, Samiha Abdel-Rahman, Ashraf Mahmoud, Ahmed Hassan: "N-bromosuccinimide/fluorescein system for spectrophotometric determination of H2-receptor antagonists in their dosage forms" Int. J. Res. Pharm. Sci., 1 (2010) 86-93.

20.  Ibrahim A. Darwish, Tarek Aboul-Fadl, Nasr Y. Khalil, Ashraf M. Mahmoud,  Abdul-Rahman M. Al-Obaid: New Sensitive HPLC Method for Evaluation of the Pharmacokinetics of New Amantadine Prodrugs as Hepatic Delivery Systems to Enhance its Activity against HCV. Int. J. Res. Pharm. Sci., 1 (2010) 151-157.

21.  Ibrahim A. Darwish, Ashraf M. Mahmoud, Abdul-Rahman A. Al-Majed: Novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis of losartan potassium. Int. J. Res. Pharm. Sci., 1 (2010) 151-157.

22.  Ibrahim A. Darwish, Ashraf M. Mahmoud, Abdul-Rahman A. Al-Majed: Novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis:  Application in the analysis of certain antihypertensive drugs. Acta Pharmaceutica, 60 (2010).

23.  Badraddin M.H. Al-Hadiya, Ashraf M. Mahmoud: Gemifloxacin Comprehensive Profile, Analytical Profile of Drug Substances, Excipients and Related Methodology, Vol. 39, 2011.

24.  Ashraf M. Mahmoud, Hanaa M. Abdel-Wadood, Niveen A. Mohamed: Kinetic Spectrophotometric Method for Determination of Amilodipine Besylate in its Pharmaceutical Tablets. J. Pharm. Anal., 2 (2012)

25.  Ashraf M. Mahmoud, Salwa R. El-Shabouri, Noha N. Atia, Wesam M. El-koussi: Novel simple HPTLC approach to avert pretreatment of plasma samples: Application to the determination of gemifloxacin mesylate using fluorescence detection. Assiut University 7th Pharmaceutical Sciences Conference, Assiut, Egypt, Marsh 14-15th , 2012

26.  Noha N. Atia, Ashraf M. Mahmoud, Salwa R. El-Shabouri, Wesam M. El-koussi: Two validated spectrofluorometric methods for determination of Gemifloxacin mesylate in tablets and human plasma. Int. J. Ana.l Chem., 2013  (Published online, 11 pages, http://dx.doi.org/10.1155/2013/137279).

27.  Marwa Fathy Bakr Ali, Naoya Kishikawa, Kaname Ohyama, Horria Abdel-Mageed Mohamed, Hanaa Mohamed Abdel-Wadood, Ashraf Mohamed Mohamed, Naotaka Kuroda, Chromatographic determination of aliphatic aldehydes in humanserum after pre-column derivatization using 2,2_-furil, a novelfluorogenic reagent. J. Chromatogr. A 1300 (2013) 199– 203.   

28.  Marwa F Ali, Naoya Kishikawa, Kaname Ohyama, Horria A. Mohamed, Hanaa M. Abdel-Wadood, Ashraf M Mahmoud, Takahiro Imazato, Yukitaka Ueki, Mitsuhiro Wada, Naotaka Kuroda: Chromatographic determination of low-molecular mass unsaturated aliphatic aldehydes with peroxyoxalate chemoluminescence detection after fluorescence labeling with4-(N,N-dimethyl-aminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole. J. Chromatography B, 953-954 (2014)147-152.

29.  Wesam M El-Koussi, Noha N Atia, Ashraf M Mahmoud, Salwa R El-Shabouri: HPTLC method for direct determination of gemifloxacin mesylate in human plasma. J. Chromatography B, 967 (2014) 98-101.

30.  Ashraf M Mahmoud, Noha N Atia, Salwa R El-Shabouri, Wesam M El-Koussi: Flow injection analysis for quantitative determination of gemifloxacin mesylate in pharmaceutical preparations and human plasma. Assiut University 9th International Pharmaceutical Sciences Conference, Assiut, Egypt, 12-13th March 2014, p. 93.

31.  Sameh Ahmed, Ashraf M Mahmoud: A novel salting-out assisted extraction coupled with HPLC- fluorescence detection for trace determination of vitamin K homologues in human plasma. Talanta 144 (2015) 480-487.

32.  Ashraf M Mahmoud, Noha N Atia, Salwa R El-Shabouri, Wesam M El-Koussi: Development and Validation of Stability Indicating HPTLC Assay for Determination of Gemifloxacin Mesylate in Dosage Forms. American J Anal Chem 6 (2015) 85-97.

33.  Ashraf M Mahmoud, Sameh Ahmed: A Validated High-Throughput Fluorometric Method for Determination of Omeprazole in Quality Control Laboratory via Charge Transfer Sensitized Fluorescence. J Fluorescence. 26 (2016) xx-xx. DOI 10.1007/s10895-015-1737-4

34.  Ashraf M. Mahmoud, Saad A AlQahtani: Spectrophotometric Study for the Reaction of Pentoxifylline Hydrochloride with 1, 2-Naphthoquinone-4-Sulphonate: Kinetics, Mechanism and Application for Development of High-throughput Kinetic Microwell Assay for Pentoxifylline in Quality Control Laboratory. American J Anal Chem 7 (2016) 179-191.

35.  Wesam M El-Koussi, Noha N Atia, Ashraf M Mahmoud: Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate. Book: 20/11/2013; Lambert Academic Publishing, ISBN: 978-3-659-4905-4.

36.  Saad A AlQahtani, Samer S. Abu-Alrub, Ashraf M. Mahmoud: Adsorption of food coloring allura red dye (E129) from aqueous solutions using activated carbon. International Journal of Food and Allied Sciences, 2017, 3(1): 10-19